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KMID : 1234420100380030335
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 3 p.335 ~ p.339
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Isolation and Characterization of a Diesel-Degrading Bacterium, Gordonia sp. SD8
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Hong Sun-Hwa
Kim Ji-Young
Cho Kyung-Suk
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Abstract
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A diesel-degrading bacterium, Gordonia sp. SD8, was isolated from soil contaminated with petroleum, and its diesel degradation was characterized in a soil as well as a liquid culture system. SD8 could grow in the mineral salt medium supplemented with diesel as a sole carbon and energy source. The maximum specific growth rate (0.67±0.05 d-1) and diesel degradation rate (1,727±145 mg-TPH L-1 d-1) of SD8 showed at 20,000 mg-TPH L-1 and 30oC, and then this bacterium could degrade high strength of diesel of 40,000 mg-TPH L-1. The residual diesel concentration in the inoculated soil with SD8 was 3,724 mg- TPH kg-dry soil-1 after 17 days, whereas the diesel concentration in the non-inoculated soil was 8,150±755 mg-TPH kg-dry soil-1. These results indicate that Gordonia sp. SD8 can serve as a promising microbial
resource for the bioremediaion of contaminated soil with petroleum hydrocarbons including diesel.
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KEYWORD
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Bioremediation, Gordonia sp., diesel, soil contamination
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